Total Synthesis of a Functional Designer Eukaryotic Chromosome

@article{Annaluru2014TotalSO,
  title={Total Synthesis of a Functional Designer Eukaryotic Chromosome},
  author={Narayana Annaluru and H{\'e}lo{\"i}se Muller and Leslie A. Mitchell and Sivaprakash Ramalingam and Giovanni Stracquadanio and Sarah M. Richardson and Jessica S. Dymond and Zheng Kuang and Lisa Scheifele and Eric M. Cooper and Yizhi Cai and Karen Zeller and Neta Agmon and Jeffrey S. Han and Michalis Hadjithomas and Jennifer Tullman and Katrina Caravelli and Kimberly M. Cirelli and Zheyuan Guo and Viktoriya London and Apurva Yeluru and Sindurathy Murugan and Karthikeyan Kandavelou and Nicolas Agier and Gilles Fischer and Kun Yang and J Andrew Martin and Murat Bilgel and Pavlo Bohutskyi and Kristin M. Boulier and Brian J. Capaldo and Joy Chang and Kristie Charoen and Woo Jin Choi and Peter Deng and James E. DiCarlo and Judy C. Doong and Jessilyn P. Dunn and Jason I. Feinberg and Christopher Fernandez and Charlotte E. Floria and David A. Gladowski and Pasha Hadidi and Isabel Emiko Ishizuka and Javaneh Jabbari and Calvin Y. L. Lau and Pablo A. Lee and Sean Li and Denise Lin and Matthias E. Linder and Jonathan Guyang Ling and Jaime Liu and Jonathan Liu and Mariya London and Henry Ma and Jessica Mao and Jessica E. McDade and Alexandra McMillan and Aaron M. Moore and Won Chan Oh and Yuxiao Ouyang and Ruchi Patel and Marina Paul and Laura C. Paulsen and Judy Qiu and Alex Rhee and Matthew G Rubashkin and Ina Y. Soh and Nathaniel E. Sotuyo and Venkatesh Srinivas and Allison Suarez and Andy Wong and Remus S Wong and Wei Rose Xie and Yijie Xu and Allen T. Yu and Romain Koszul and Joel S. Bader and Jef D. Boeke and Srinivasan Chandrasegaran},
  journal={Science},
  year={2014},
  volume={344},
  pages={55 - 58}
}
Designer Chromosome One of the ultimate aims of synthetic biology is to build designer organisms from the ground up. Rapid advances in DNA synthesis has allowed the assembly of complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge to synthetic biologists. Annaluru et al. (p. 55, published online 27 March) designed a synthetic eukaryotic chromosome based on yeast chromosome III. The designer chromosome, shorn… Expand
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