Total RNA isolation from stallion sperm and testis biopsies.

  title={Total RNA isolation from stallion sperm and testis biopsies.},
  author={Pranab J Das and Nandina Paria and Ashley L Gustafson-Seabury and Monika Vishnoi and Sankar Prasad Chaki and Charles C. Love and Dickson D Varner and Bhanu P. Chowdhary and T Raudsepp},
  volume={74 6},
  pages={1099-1106, 1106e1-2}
Sperm mRNA transcriptional profiles can be used to evaluate male fertility, yet differences between species in sperm attributes and packaging require adjustments in sperm RNA isolation protocols. The objective was to optimize RNA isolation methodology for fresh, frozen, and extended ejaculates, and epididymal sperm of stallions. Additionally, a protocol for RNA isolation from testis biopsies was established. Separation of sperm from somatic cells was critical for assuring the isolation of sperm… CONTINUE READING


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Amplification with PTPRC. Lanes: 1–5 – five different reverse transcriptase and water; 8 – male genomic DNA; 9 – female with P ol with Identification of ten novel genes involved in human spermato

  • YH Lin, YM Lin, YN Teng, TY Hsieh, YS Lin, PL. Kuo
  • Theriogenology
  • 2010
2 Excerpts

Semen processing for the subfertile stallion

  • DD Varner, CC Love, SP Brinsko, TL Blanchard, SB Bliss
  • J Equine Vet Sci
  • 2008

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