Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: Analogous measurements in insect and human cells

@article{Sinden1980TorsionalTI,
  title={Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: Analogous measurements in insect and human cells},
  author={R. Sinden and J. Carlson and D. Pettijohn},
  journal={Cell},
  year={1980},
  volume={21},
  pages={773-783}
}
  • R. Sinden, J. Carlson, D. Pettijohn
  • Published 1980
  • Biology, Medicine
  • Cell
    • The rate of covalent photobinding of trimethylpsoralen to DNA is greater when the DNA is wound with negative superhelical tension than when it is relaxed. In vitro the rate of photobinding is directly proportional to the negative superhelical density of the DNA. Thus measurement of the rate of photobinding provides an assay for probing in vivo unrestrained tension in the winding of the DNA double helix. This approach has been applied to measure torsional tension in DNA as it is packaged in… CONTINUE READING
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    References

    SHOWING 1-10 OF 48 REFERENCES
    Cross-linking and relaxation of supercoiled DNA by psoralen and light.
    • 14
    Superhelical Escherichia coli DNA: relaxation by coumermycin.
    • 145
    Thermodynamic properties of superhelical DNAs.
    • 158
    DNA unwinding induced by photoaddition of psoralen derivatives and determination of dark-binding equilibrium constants by gel electrophoresis.
    • G. Wiesehahn, J. Hearst
    • Chemistry, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1978
    • 38
    • PDF
    E. coli DNA binding protein HU forms nucleosome-like structure with circular double-stranded DNA
    • 365
    DNA-DNA gyrase complex: the wrapping of the DNA duplex outside the enzyme
    • 147
    Type II DNA topoisomerases: Enzymes that can unknot a topologically knotted DNA molecule via a reversible double-strand break
    • 437
    • PDF
    Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.
    • W. Keller
    • Mathematics, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1975
    • 331
    • PDF
    Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster
    • 430
    Micrococcus luteus DNA gyrase: active components and a model for its supercoiling of DNA.
    • L. Liu, J. Wang
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1978
    • 145
    • PDF