Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: Analogous measurements in insect and human cells

@article{Sinden1980TorsionalTI,
  title={Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: Analogous measurements in insect and human cells},
  author={R. Sinden and J. Carlson and D. Pettijohn},
  journal={Cell},
  year={1980},
  volume={21},
  pages={773-783}
}
  • R. Sinden, J. Carlson, D. Pettijohn
  • Published 1980
  • Biology, Medicine
  • Cell
  • The rate of covalent photobinding of trimethylpsoralen to DNA is greater when the DNA is wound with negative superhelical tension than when it is relaxed. In vitro the rate of photobinding is directly proportional to the negative superhelical density of the DNA. Thus measurement of the rate of photobinding provides an assay for probing in vivo unrestrained tension in the winding of the DNA double helix. This approach has been applied to measure torsional tension in DNA as it is packaged in… CONTINUE READING
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