Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus.

@article{Lemak2013ToroidalSA,
  title={Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus.},
  author={Sofia Lemak and Natalia Beloglazova and Boguslaw P. Nocek and Tatiana Skarina and Robert L Flick and Greg A. Brown and Ana Popovic and Andrzej Joachimiak and Alexei Savchenko and Alexander F Yakunin},
  journal={Journal of the American Chemical Society},
  year={2013},
  volume={135 46},
  pages={17476-87}
}
Cas4 proteins, a core protein family associated with the microbial system of adaptive immunity CRISPR, are predicted to function in the adaptation step of the CRISPR mechanism. Here we show that the Cas4 protein SSO0001 from the archaeon Sulfolobus solfataricus has metal-dependent endonuclease and 5'→3' exonuclease activities against single-stranded DNA, as well as ATP-independent DNA unwinding activity toward double-stranded DNA. The crystal structure of SSO0001 revealed a decameric toroid… CONTINUE READING

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