Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae.

Abstract

Tor1p and Tor2p kinases, targets of the immune-suppressive antibiotic rapamycin, are components of a highly conserved signaling network that couples nutrient availability and cell growth. To gain insight into the molecular basis underlying Tor-dependent signaling, we used cell fractionation and immunoaffinity chromatography to examine the physical environment of Tor2p. We found that the majority of Tor2p associates with a membrane-bound compartment along with at least four other proteins, Avo1p-Avo3p and Lst8p. Using immunogold electron microscopy, we observed that Tor2p, as well as Tor1p, localizes in punctate clusters to regions adjacent to the plasma membrane and within the cell interior, often in association with characteristic membranous tracks. Cell fractionation, coimmunoprecipitation, and immunogold electron microscopy experiments confirmed that Lst8 associates with both Tor2p as well as Tor1p at these membranous sites. In contrast, we find that Kog1, the yeast homologue of the mammalian Tor regulatory protein Raptor, interacts preferentially with Tor1p. These findings provide evidence for the existence of Tor signaling complexes that contain distinct as well as overlapping components. That these complexes colocalize to a membrane-bound compartment suggests an intimate relationship between membrane-mediated signaling and Tor activity.

Extracted Key Phrases

7 Figures and Tables

050'04'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

468 Citations

Semantic Scholar estimates that this publication has 468 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Wedaman2003TorKA, title={Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae.}, author={Karen P. Wedaman and Aaron W Reinke and Scott D. Anderson and John Yates and J . Michael McCaffery and Ted Powers}, journal={Molecular biology of the cell}, year={2003}, volume={14 3}, pages={1204-20} }