Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq

@article{Dominissini2012TopologyOT,
  title={Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq},
  author={Dan Dominissini and Sharon Moshitch-Moshkovitz and Schraga Schwartz and Mali Salmon-Divon and Lior Ungar and Sivan Osenberg and Karen Cesarkas and Jasmine Jacob-Hirsch and Ninette Amariglio and Martin Kupiec and Rotem Sorek and Gideon Rechavi},
  journal={Nature},
  year={2012},
  volume={485},
  pages={201-206}
}
An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. [] Key Result We identify over 12,000 m6A sites characterized by a typical consensus in the transcripts of more than 7,000 human genes. Sites preferentially appear in two distinct landmarks—around stop codons and within long internal exons—and are highly conserved between human and mouse.

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TLDR
It is reported that recent work has shown RNA modifications like m6A, collectively called the “epitranscriptome,” are a pervasive feature of mammalian cells and likely play a role in development and disease.

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TLDR
The advances, open questions, and intriguing possibilities in this emerging field that might be called RNA modomics or epitranscriptomics are summarized.

Dynamic landscape and evolution of m6A methylation in human

TLDR
A resource of human m6A profile is provided for future studies of m 6A functions, and a role ofm6A modification in human evolutionary adaptation and disease susceptibility is suggested.

Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation

TLDR
It is revealed that m7G is enriched at the 5′UTR region and AG-rich contexts, a feature that is well-conserved across different human/mouse cell lines and mouse tissues, and highlighted as a novel epitranscriptomic marker with regulatory roles in translation.

Single-nucleotide resolution mapping of m6A and m6Am throughout the transcriptome

TLDR
m6A individual-nucleotide-resolution cross-linking and immunoprecipitation (miCLIP) is developed and used to demonstrate that antibodies to m6A can induce specific mutational signatures at m 6A residues after ultraviolet light–induced antibody-RNA cross- linking and reverse transcription.

m6A: A novel hallmark of translation

TLDR
The elucidation of the mechanism underlying stress-induced 5’UTR methylation was discovered and several surprising features of the mA "reader" YTHDF2 were discovered, including the fact that mA resembles mG, and it was speculated that the stress- induced 5‘UTRmethylation permits the translation machinery to identify stressinduced messages from preexisting mRNAs, thereby achieving selective translation.

Genome-wide identification of mRNA 5-methylcytosine in mammals

TLDR
A quantitative atlas of RNA m5C sites in human and mouse tissues based on a new discovery pipeline allows the identification of sequence motifs and structural features associated with the modification and provides a resource for future studies.

m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome.

TLDR
m6A-SAC-seq is a quantitative method to dissect the dynamics and functional roles of m6A sites in diverse biological processes using limited input RNA.

Most m6A RNA Modifications in Protein‐Coding Regions Are Evolutionarily Unconserved and Likely Nonfunctional

TLDR
It is suggested that most m6A modifications in protein‐coding regions are nonfunctional and nonadaptive, probably resulting from off‐target activities of m6a methyltransferases.
...

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