Topological alteration of the CYP3A4 active site by the divalent cation Mg(2+).

@article{Schrag2000TopologicalAO,
  title={Topological alteration of the CYP3A4 active site by the divalent cation Mg(2+).},
  author={Michael L Schrag and Larry C. Wienkers},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2000},
  volume={28 10},
  pages={1198-201}
}
Phenyldiazene reacted with lymphoblast-expressed CYP3A4 to give a stable phenyl-iron complex that could be induced to rearrange in situ producing approximately equal amounts of four N-phenyl-protoporphyrin IX isomers (N(B):N(A):N(C):N(D), 01:01:02:02). In the presence of 10 mM MgCl(2), the formation profile of the protoporphyrin isomers was markedly altered compared with control, favoring the N(A) isomer (N(B):N(A):N(C):N(D), 01:34:01:02). In addition, an investigation of MgCl(2) effects on… CONTINUE READING

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