Topological Selectivity in Xer Site-Specific Recombination

  title={Topological Selectivity in Xer Site-Specific Recombination},
  author={Sean Colloms and Jonathan Bath and David J Sherratt},
The product topology of Xer-mediated site-specific recombination at plasmid sites has been determined. The product of deletion at pSC101 psi is a right-handed antiparallel 4-noded catenane. The ColE1 cer deletion product has an identical topology, except that only one pair of strands is exchanged. These specific product topologies imply that the productive synaptic complex and the strand exchange mechanism have fixed topologies. Further analysis suggests that synapsis traps exactly three… CONTINUE READING

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