Topological Selectivity in Xer Site-Specific Recombination

@article{Colloms1997TopologicalSI,
  title={Topological Selectivity in Xer Site-Specific Recombination},
  author={Sean Colloms and Jonathan Bath and David J Sherratt},
  journal={Cell},
  year={1997},
  volume={88},
  pages={855-864}
}
The product topology of Xer-mediated site-specific recombination at plasmid sites has been determined. The product of deletion at pSC101 psi is a right-handed antiparallel 4-noded catenane. The ColE1 cer deletion product has an identical topology, except that only one pair of strands is exchanged. These specific product topologies imply that the productive synaptic complex and the strand exchange mechanism have fixed topologies. Further analysis suggests that synapsis traps exactly three… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 39 references

Large-scale preparation of T4 endonuclease VII from over-expressing bacteria.

European journal of biochemistry • 1990
View 10 Excerpts
Highly Influenced

Geometric arrangein 50 mM Tris – HCl ( pH 8 . 0 ) , 10 mM MgCl 2 , 50 mM NaCl , 0 . 3 mg / ml ments of Tn 3 resolvase sites

K. R. Benjamin, A. P. Abola, R. Kanaar, N. R. Cozzarelli
J . Biol . Chem . • 1996
View 1 Excerpt

Topological selectivity in site - specific recombination

W. M. Stark, M. R. Boocock
Mobile Genetic Elements • 1995
View 1 Excerpt

E . coli topoisomerase IV : purification , characterization , subunit structure and subunit interactions

H. Peng, K. J. andMarians
J . Biol . Chem . • 1993

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