Topoisomerase II-mediated breaks in spermatozoa cause the specific degradation of paternal DNA in fertilized oocytes.

@article{Yamauchi2007TopoisomeraseIB,
  title={Topoisomerase II-mediated breaks in spermatozoa cause the specific degradation of paternal DNA in fertilized oocytes.},
  author={Yasuhiro Yamauchi and Jeffrey A Shaman and W Steven Ward},
  journal={Biology of reproduction},
  year={2007},
  volume={76 4},
  pages={666-72}
}
We have demonstrated that mouse spermatozoa can cleave their DNA into 50-kb fragments when treated with Triton X-100, MnCl(2), and CaCl(2). This cleavage, which is termed sperm chromatin fragmentation (SCF), is mediated by topoisomerase IIB (TOP2B) following stimulation by a factor in the epididymal fluid, most likely a nuclease, and can be at least partially religated by EDTA. When the protamines are removed, this DNA breakage is followed by digestion of the DNA by a nuclease(s). We tested… CONTINUE READING

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