Topographical analysis of the plasma membrane-associated sucrose binding protein from soybean.

@article{Overvoorde1994TopographicalAO,
  title={Topographical analysis of the plasma membrane-associated sucrose binding protein from soybean.},
  author={Paul J Overvoorde and Howard Dean Grimes},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 21},
  pages={15154-61}
}
Plasma membranes of soybean cells actively engaged in sucrose transport have a sucrose binding protein (SBP) that does not appear to be an integral membrane protein. Experiments were undertaken to analyze the topographical association of this protein with the membrane. Treatment of purified plasma membrane vesicles with either 1 M KCl or KI released less than 35% of the sucrose binding protein from the membrane whereas treatment with either 4 M urea or 0.1 M Na2CO3, pH 11.5, disassociated… CONTINUE READING