Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo.

@article{Pftzner2004TopicalCS,
  title={Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo.},
  author={Wolfgang Pf{\"u}tzner and Jonathan C. Vogel},
  journal={Cells, tissues, organs},
  year={2004},
  volume={177 3},
  pages={
          151-9
        }
}
In gene therapy, a clinically relevant therapeutic effect requires long-term expression of the desired gene at a level sufficient to correct or at least alleviate the underlying gene defect. One approach to achieve persistent as well as high-level transgene expression in a significant percentage of target cells would be to select cells expressing both the desired transgene and a linked selectable gene--such as the human multi-drug resistance (MDR1) gene--in a bicistronic vector. Because of its… CONTINUE READING

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