Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo.

Abstract

For skin gene therapy, achieving prolonged high-level gene expression in a significant percentage of keratinocytes (KC) is difficult because we cannot selectively target KC stem cells. We now demonstrate that topical colchicine treatment can be used to select, in vivo, KC progenitor cells transduced with the multidrug resistance gene (MDR1). When human skin… (More)

Topics

5 Figures and Tables

Cite this paper

@article{Pfutzner2002TopicalCS, title={Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo.}, author={Wolfgang Pfutzner and Atshushi Terunuma and Christine L Tock and E K Snead and Tadeusz M. Kolodka and Malcolm Gottesman and L Susan Taichman and Johannes C. Vogel}, journal={Cells, tissues, organs}, year={2002}, volume={177 3}, pages={151-9} }