Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation.

@article{Bermejo2007Top1AT,
  title={Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation.},
  author={Rodrigo Bermejo and Ylli Doksani and Thelma Capra and Yuki-Mori Katou and Hirokazu Tanaka and Katsuhiko Shirahige and Marco Foiani},
  journal={Genes & development},
  year={2007},
  volume={21 15},
  pages={1921-36}
}
DNA topoisomerases solve topological problems during chromosome metabolism. We investigated where and when Top1 and Top2 are recruited on replicating chromosomes and how their inactivation affects fork integrity and DNA damage checkpoint activation. We show that, in the context of replicating chromatin, Top1 and Top2 act within a 600-base-pair (bp) region spanning the moving forks. Top2 exhibits additional S-phase clusters at specific intergenic loci, mostly containing promoters. TOP1 ablation… CONTINUE READING

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