Top down proteomics of human membrane proteins from enriched mitochondrial fractions.

@article{Catherman2013TopDP,
  title={Top down proteomics of human membrane proteins from enriched mitochondrial fractions.},
  author={Adam D. Catherman and Mingxi Li and John C Tran and Kenneth R. Durbin and Philip D. Compton and Bryan P. Early and Paul M Thomas and Neil L. Kelleher},
  journal={Analytical chemistry},
  year={2013},
  volume={85 3},
  pages={1880-8}
}
The interrogation of intact integral membrane proteins has long been a challenge for biological mass spectrometry. Here, we demonstrate the application of top down mass spectrometry to whole membrane proteins below 60 kDa with up to 8 transmembrane helices. Analysis of enriched mitochondrial membrane preparations from human cells yielded identification of 83 integral membrane proteins, along with 163 membrane-associated or soluble proteins, with a median q value of 3 × 10(-10). An analysis of… CONTINUE READING
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