Tonoplast-located GmCLC1 and GmNHX1 from soybean enhance NaCl tolerance in transgenic bright yellow (BY)-2 cells.

@article{Li2006TonoplastlocatedGA,
  title={Tonoplast-located GmCLC1 and GmNHX1 from soybean enhance NaCl tolerance in transgenic bright yellow (BY)-2 cells.},
  author={W. F. Li and Fuk-Ling Wong and Sau-na Tsai and Tsui-Hung Phang and Guihua Shao and Hon-Ming Lam},
  journal={Plant, cell & environment},
  year={2006},
  volume={29 6},
  pages={1122-37}
}
Genes encoding ion transporters that regulate ion homeostasis in soybean have not been carefully investigated. Using degenerate primers, we cloned a putative chloride channel gene (GmCLC1) and a putative Na+/H+ antiporter gene (GmNHX1) from soybean. Confocal microscopic studies using yellow fluorescent fusion proteins revealed that GmCLC1 and GmNHX1 were both localized on tonoplast. The expressions of GmCLC1 and GmNHX1 were both induced by NaCl or dehydration stress imposed by polyethylene… CONTINUE READING