Tonic regulation of excitation-contraction coupling by basal protein kinase C activity in isolated cardiac myocytes.

@article{Nicolas1998TonicRO,
  title={Tonic regulation of excitation-contraction coupling by basal protein kinase C activity in isolated cardiac myocytes.},
  author={J. M. Nicolas and Dominique C Renard-Rooney and A. P. Thomas},
  journal={Journal of molecular and cellular cardiology},
  year={1998},
  volume={30 12},
  pages={2591-604}
}
A high-speed imaging technique was used to investigate the effects of inhibitors and activators of protein kinase C (PKC) on the [Ca2+]i transients and contraction of fura-2 loaded rat ventricular cardiac myocytes. The amplitude of the [Ca2+]i transient was reduced following treatment with 100 nM phorbol 12,13-dibutyrate (PDBu), whereas the PKC inhibitors staurosporine (0.5 microM) and calphostin C (10 microM) increased [Ca2+]i transient amplitude, elevated basal [Ca2+]i and slowed the decay of… CONTINUE READING