Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris.

@article{Mogelsvang2003TomographicEF,
  title={Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris.},
  author={Soren Mogelsvang and Natalia Gomez-Ospina and Jon Soderholm and Benjamin S. Glick and L Andrew Staehelin},
  journal={Molecular biology of the cell},
  year={2003},
  volume={14 6},
  pages={
          2277-91
        }
}
The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic reticulum (tER) sites, making this organism ideal for structure-function studies of the secretory pathway. Here, we have used P. pastoris to test various models for Golgi trafficking. The experimental approach was to analyze P. pastoris tER-Golgi units by using cryofixed and freeze-substituted cells for electron microscope tomography, immunoelectron microscopy, and serial thin section… CONTINUE READING

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