Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris.

  title={Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris.},
  author={Soren Mogelsvang and Natalia Gomez-Ospina and Jon Soderholm and Benjamin S. Glick and L Andrew Staehelin},
  journal={Molecular biology of the cell},
  volume={14 6},
The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic reticulum (tER) sites, making this organism ideal for structure-function studies of the secretory pathway. Here, we have used P. pastoris to test various models for Golgi trafficking. The experimental approach was to analyze P. pastoris tER-Golgi units by using cryofixed and freeze-substituted cells for electron microscope tomography, immunoelectron microscopy, and serial thin section… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 54 extracted citations


Publications referenced by this paper.
Showing 1-10 of 74 references

puter visualization of three - dimensional image data using IMOD

  • M. S. Ladinsky, D. N. Mastronarde, J. R. McIntosh, K. E. Howell, L. A. Staehelin
  • J . Struct . Biol .
  • 1999
Highly Influential
14 Excerpts

Three-dimensional structure of the Golgi apparatus in mammalian cells

  • A. Rambourg, Y. Clermont
  • The Golgi Apparatus,
  • 1997
Highly Influential
3 Excerpts

Development of the

  • S. J. Gould, D. McCollum, A. P. Spong, J. A. Heyman
  • 1992
Highly Influential
4 Excerpts

Similar Papers

Loading similar papers…