Tomato Transformation and Transgenic Plant Production

  title={Tomato Transformation and Transgenic Plant Production},
  author={Carolina Cortina and Francisco A. Culi{\'a}{\~n}ez-maci{\`a}},
  journal={Plant Cell, Tissue and Organ Culture},
Tomato transformation and regeneration were analysed and optimized. Cotyledon explants from Lycopersicon esculentum cv. UC82B, were infected by Agrobacterium tumefaciens strain LBA4404 harbouring the neomycin phosphotransferase (NPTII) reporter gene. The effects of phenolic compounds, vitamins and growth regulators on plant transformation and regeneration were studied. Increasing the vitamin thiamine concentration from 0.1 mg l−1 in standard medium to 0.4 mg l−1 decreased the chlorophyll lost… CONTINUE READING
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