Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays.

@article{Erenpreiss2004ToluidineBC,
  title={Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays.},
  author={Juris Erenpreiss and Katarina Jepson and A Giwercman and Igor Tsarev and J. A. Erenpreisa and Marcello Span{\^o}},
  journal={Human reproduction},
  year={2004},
  volume={19 10},
  pages={2277-82}
}
BACKGROUND Sperm DNA integrity (SDI) is an important factor in the prognosis of male fertility. Here we compare the toluidine blue (TB) image cytometry test, recently proposed by us for SDI assessment, with two other tests-the sperm chromatin structure assay (SCSA) and the terminal nick-end labelling (TUNEL) assay. METHODS Sperm samples from 35 men were evaluated for standard sperm parameters and subjected to the TB test and SCSA. Eighteen of the 35 samples were also subjected to the TUNEL… CONTINUE READING