Tolerance to Oxidative Stress in Budding Yeast by Heterologous Expression of Catalases A and T from Debaryomyces hansenii.

  title={Tolerance to Oxidative Stress in Budding Yeast by Heterologous Expression of Catalases A and T from Debaryomyces hansenii.},
  author={James Gonz{\'a}lez and Rom{\'a}n Castillo and Miguel Angel Garcia-Campos and Diego Noriega-Samaniego and Viviana Escobar-S{\'a}nchez and Lucero Romero-Aguilar and Luisa Alba-Lois and Claudia Segal-Kischinevzky},
  journal={Current microbiology},
The function of catalases A and T from the budding yeast Saccharomyces cerevisiae (ScCta1 and ScCtt1) is to decompose hydrogen peroxide (H2O2) to mitigate oxidative stress. Catalase orthologs are widely found in yeast, suggesting that scavenging H2O2 is crucial to avoid the oxidative damage caused by reactive oxygen species (ROS). However, the function of catalase orthologs has not yet been experimentally characterized in vivo. Here, we heterologously expressed Debaryomyces hansenii DhCTA1 and… 
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The results suggest that catalase is not essential for yeast cells under normal conditions, but plays an important role in the acquisition of tolerance to oxidative stress in the adaptive response of these cells.
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Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii
The results demonstrate that DhGzf3 can play a role as a negative GATA transcription factor when expressed in S. cerevisiae and that it most probably represents the only member of this family in D. hansenii, which point to the Gata transcription factors as relevant elements for alkaline-pH tolerance.
Debaryomyces hansenii, a highly osmo-tolerant and halo-tolerant yeast, maintains activated Dhog1p in the cytoplasm during its growth under severe osmotic stress
Evidence is provided that D. hansenii maintains phosphorylated Dhog1p in the cytoplasm during its growth under severe osmotic stress, and it is demonstrated that the pathway is activated by high osmolarity, oxidative and UV stress but not by heat stress.
Contribution of the mitogen-activated protein kinase Hog1 to the halotolerance of the marine yeast Debaryomyces hansenii
It was found that DhHog1 can regulate response to alkali stress during hyperosmotic conditions and that it plays a role in oxidative and endoplasmic reticulum stress, which provides new insight into the contribution of this MAPK in halotolerance of this yeast.
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It is demonstrated that both stresses are connected by the generation of intracellular ROS, and that hydrogen peroxide can affect the accumulation of in-cell sodium.
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Transient adaptation to oxidative stress in yeast.
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