Toehold-initiated rolling circle amplification for visualizing individual microRNAs in situ in single cells.

@article{Deng2014ToeholdinitiatedRC,
  title={Toehold-initiated rolling circle amplification for visualizing individual microRNAs in situ in single cells.},
  author={Ruijie Deng and Longhua Tang and Qianqian Tian and Ying Min Wang and Lei Lin and Jinghong Li},
  journal={Angewandte Chemie},
  year={2014},
  volume={53 9},
  pages={2389-93}
}
The ability to quantitate and visualize microRNAs (miRNAs) in situ in single cells would greatly facilitate the elucidation of miRNA-mediated regulatory circuits and their disease associations. A toehold-initiated strand-displacement process was used to initiate rolling circle amplification of specific miRNAs, an approach that achieves both stringent recognition and in situ amplification of the target miRNA. This assay, termed toehold-initiated rolling circle amplification (TIRCA), can be… CONTINUE READING
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