Tocopherol contents of lipoproteins from frozen plasma separated by affinity chromatography

  title={Tocopherol contents of lipoproteins from frozen plasma separated by affinity chromatography},
  author={Beverly A. Clevidence and Rachel Ballard-Barbash},
We investigated whether freezing and storage of plasma altered α-tocopherol levels of whole plasma or the lipoprotein fractions derived from such plasma. Plasma from 24 men, at each of two collection periods, was frozen at −20°C for six weeks, then high-density lipoproteins (HDL) were separated from low- plus very low-density lipoproteins (LDL-VLDL) by heparin affinity chromatography. Whole plasma and the lipoprotein fractions were analyzed for α-tocopherol content and compared to counterparts… CONTINUE READING


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