Tobacco streak virus (strain dahlia) suppresses post-transcriptional gene silencing of flavone synthase II in black dahlia cultivars and causes a drastic flower color change

  title={Tobacco streak virus (strain dahlia) suppresses post-transcriptional gene silencing of flavone synthase II in black dahlia cultivars and causes a drastic flower color change},
  author={Ayumi Deguchi and Fumi Tatsuzawa and Munetaka Hosokawa and Motoaki Doi and Sho Ohno},
AbstractMain conclusionTobacco streak virus suppressed post-transcriptional gene silencing and caused a flower color change in black dahlias, which supported the role of cyanidin-based anthocyanins for black flower appearance. Black flower color of dahlia (Dahlia variabilis) has been attributed, in part, to the high accumulation of cyanidin-based anthocyanins that occurs when flavone synthesis is reduced because of post-transcriptional gene silencing (PTGS) of flavone synthase II (DvFNS… 
Understanding Functional Genomics of PTGS Silencing Mechanisms for Tobacco Streak Virus and Other Ilarviruses Mediated by RNAi and VIGS
Post-transcriptional gene silencing (PTGS) is a successful technology for the investigation of functions of gene in plants and can be achieved either by RNA interference (RNAi) or virus-induced genesilencing (VIGS).
CmMYB9a activates floral coloration by positively regulating anthocyanin biosynthesis in chrysanthemum
This study functionally characterizes the role of CmMYB9a in regulating the branched pathways of flavonoids in chrysanthemum flowers and suggests that the transcription factor facilitates metabolic flux into anthocyanin and flavone biosynthesis.
A blackish-flowered cultivar of Catharanthus roseus accumulates high concentrations of a novel anthocyanin with a unique feature of aggregation in weak acid solutions
Abstract A cultivar of Catharanthus roseus, ‘Jams ‘N Jellies Blackberry’, was investigated for its rare blackish flower color by comparing several factors affecting flower color with another ten C.
Studies on the mechanisms of pathogenic changes of plant viruses
  • S. Kuwata
  • Biology
    Journal of General Plant Pathology
  • 2018
Research results on the pathogenicity of cucumber mosaic virus (CMV) and tobacco streak virus (TSV) are introduced, which suggest that viral symptoms were altered by nucleotide substitutions in genomic RNA and/or amino acid changes in viral proteins.
Quantitative Evaluation of the Contribution of Four Major Anthocyanins to Black Flower Coloring of Dahlia Petals
Anthocyanins to Black Flower Coloring of Dahlia Petals Author(s) Deguchi, Ayumi; Tatsuzawa, Fumi; Hosokawa, Munetaka; Doi, Motoaki; Ohno, Sho Citation The Horticulture Journal (2016), 85(4): 340-350


Endogenous post-transcriptional gene silencing of flavone synthase resulting in high accumulation of anthocyanins in black dahlia cultivars
A comparative study using black and purple cultivars revealed that neither the absence of flavones nor high accumulation of total anthocyanidins is solely sufficient for black appearance, but that cyanidin content in petals is also an important factor in the phenotype.
Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia
It is concluded that simultaneous siRNA-mediated post-transcriptional gene silencing of redundant CHS genes results in the appearance of pure white color in dahlias.
Patterning of Virus-Infected Glycine max Seed Coat Is Associated with Suppression of Endogenous Silencing of Chalcone Synthase Genes
It is demonstrated that naturally occurring PTGS plays a key role in expression of a distinctive phenotype in plants and is presented as a simple clear example of the elucidation of the molecular mechanism for viral symptom induction.
A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)
Dahlias (Dahlia variabilis) exhibit a wide range of flower colours because of accumulation of anthocyanin and other flavonoids in their ray florets. Two lateral mutants were used that spontaneously
'Le Rouge et le Noir': A decline in flavone formation correlates with the rare color of black dahlia (Dahlia variabilis hort.) flowers
In the majority of black cultivars this is due to decreased flavone accumulation and thus a lack of competition for flavanones as the common precursors of flavone formation and the anthocyanin pathway, which establishes the black flowering phenotypes.
Retracted: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana
By expressing PVY and CMV‐encoded proteins in a PVX vector, it is shown that the viral suppressors of gene silencing are the HCPro of PVy and the 2b protein of CMV.
Virus-specific spatial differences in the interference with silencing of the chs-A gene in non-transgenic petunia.
The results show that virus infection can interfere with PTGS of a native plant gene, and that this can have profound effects on symptom expression.
Unraveling the Biochemical Base of Dahlia Flower Coloration
This work investigated 198 cultivars for the presence of flavonoid enzymes and found that white cultivars were frequently characterized by the lack of DFR activity, whereas in many yellow cultivars neither FHT nor D FR activity could be shown.
Temperature-dependent symptom recovery in Nicotiana benthamiana plants infected with tomato ringspot virus is associated with reduced translation of viral RNA2 and requires ARGONAUTE 1.
It is shown that recovery of Nicotiana benthamiana plants infected with the tomato ringspot virus (ToRSV) is associated with a reduction of the steady-state levels of RNA2-encoded coat protein (CP) and movement protein but not of RNA1, and this suggests an Ago1-dependent mechanism that represses the translation of viral RNA2.
A basic helix-loop-helix transcription factor DvIVS determines flower color intensity in cyanic dahlia cultivars
Results suggested that the genotype of the promoter region in DvIVS is one of the key factors determining the flower color intensity.