Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes

@article{Borisjuk2000TobaccoRD,
  title={Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes},
  author={Nikolai V. Borisjuk and Ludmyla G. Borisjuk and Slavko Komarnytsky and Sofia Timeva and Vera Hemleben and Yuri Yu. Gleba and Ilya Raskin},
  journal={Nature Biotechnology},
  year={2000},
  volume={18},
  pages={1303-1306}
}
Here we show that the cis-acting genetic element aps (amplification-promoting sequence), isolated from the nontranscribed spacer region of tobacco ribosomal DNA (rDNA), increases the level of expression of recombinant proteins. Transgenic tobacco plants, transformed with expression cassettes containing the herbicide-resistant acetolactate synthase (hr-ALS) gene or the green fluorescent protein (GFP) gene fused to the aps sequence, had greater levels of corresponding messenger RNAs (mRNAs) and… CONTINUE READING