Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana

@article{SenthilKumar2014TobaccoRV,
  title={Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana},
  author={Muthappa Senthil-Kumar and Kirankumar S Mysore},
  journal={Nature Protocols},
  year={2014},
  volume={9},
  pages={1549-1562}
}
Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) is widely used in various plant species to downregulate the expression of a target plant gene. TRV is a bipartite, positive-strand RNA virus with the TRV1 and TRV2 genomes. To induce post-transcriptional gene silencing (PTGS), the TRV2 genome is genetically modified to carry a fragment of the target gene and delivered into the plant (along with the TRV1 genome) by agroinoculation. TRV1- and TRV2-carrying Agrobacterium strains… CONTINUE READING