Tobacco mosaic virus RNA enters chloroplasts in vivo.

  title={Tobacco mosaic virus RNA enters chloroplasts in vivo.},
  author={James E Schoelz and Milton Zaitlin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={86 12},
Several lines of evidence are presented to allow us to conclude that tobacco mosaic virus (TMV) RNA enters the chloroplast in vivo. Chloroplasts were prepared from either directly inoculated or systemically infected leaves of tobacco plants inoculated with one of several strains of the virus and from uninfected control plants. Intact chloroplasts were isolated on Percoll gradients and treated with pancreatic RNase and thermolysin to destroy potential TMV virions and RNA on the outside or bound… CONTINUE READING