Tn5 insertion specificity is not influenced by IS50 end sequences in target DNA

Abstract

The bacterial transposon Tn5 inserts into dozens of sites in a gene, some of which are used preferentially (hotspots). Features of certain sites and precedents provided by several other transposons had suggested that sequences in target DNA corresponding to the ends of Tn5 or of its component IS50 elements might facilitate transposition to these sites. We… (More)
DOI: 10.1007/BF00282482

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