Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha.

Abstract

Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the inability to grow with reduced sulfur… (More)

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@article{Chandra1986Tn5inducedMA, title={Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha.}, author={T Sarat Chandra and Cornelius G. Friedrich}, journal={Journal of bacteriology}, year={1986}, volume={166 2}, pages={446-52} }