Tn5/IS50 target recognition.

@article{Goryshin1998Tn5IS50TR,
  title={Tn5/IS50 target recognition.},
  author={Igor Yu Goryshin and Joanna A. Miller and Yury V. Kil and Vladislav A. Lanzov and W. S. Reznikoff},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 18},
  pages={10716-21}
}
This communication reports an analysis of Tn5/IS50 target site selection by using an extensive collection of Tn5 and IS50 insertions in two relatively small regions of DNA (less than 1 kb each). For both regions data were collected resulting from in vitro and in vivo transposition events. Since the data sets are consistent and transposase was the only protein present in vitro, this demonstrates that target selection is a property of only transposase. There appear to be two factors governing… CONTINUE READING

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