Titration of human-bovine rotavirus reassortants using a tetrazolium-based colorimetric end-point dilution assay.

Abstract

A colorimetric end-point dilution assay was developed for the titration of rotavirus-containing samples that uses commercially available tetrazolium dyes as an indicator of virus infection. This assay offers several advantages over both plaque assays and traditional end-point dilution methods. The latter assays require manual counting of plaques or the… (More)

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