Titration of avirulent Newcastle disease virus by the plaque assay method.

@article{Kournikakis1988TitrationOA,
  title={Titration of avirulent Newcastle disease virus by the plaque assay method.},
  author={Bill Kournikakis and Jenny Fildes},
  journal={Journal of virological methods},
  year={1988},
  volume={20 4},
  pages={285-93}
}
Parameters of a plaque assay for avirulent strains of Newcastle disease virus (NDV) were optimized for reproducibility and optimum titer in LLC-MK2 cells. Plaques were visible after 2 days and maximum virus titers were reached in 3 days. Virus titers were not affected by continued incubation through 6 days, although plaque size increased. An adsorption volume of 0.1 or 0.2 ml per 60 mm Petri dish was optimal, as was an adsorption time of 45 min. Trypsin (2.5 micrograms/ml) and magnesium sulfate… CONTINUE READING

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