Titration of a CD45-FITC conjugate to determine the linearity and dynamic range of fluorescence intensity measurements on lymphocytes.

@article{Powell1998TitrationOA,
  title={Titration of a CD45-FITC conjugate to determine the linearity and dynamic range of fluorescence intensity measurements on lymphocytes.},
  author={Michael K. Powell and William G.F. Whitfield and Doug Redelman and L. Omar Henderson and Robert F. Vogt},
  journal={Cytometry},
  year={1998},
  volume={33 2},
  pages={219-24}
}
To produce biologic calibrators for relative fluorescence intensity (RFI) measurements, we stained leukocytes with serial dilutions of CD45-FITC conjugate and processed them using our regular whole blood lysis procedure. Cells were stained with conjugate concentrations ranging from twice recommended to a million-fold lower. At the highest concentrations of… CONTINUE READING