Titration of Protonable Residues in Proteins by Flash Induced H+-Release from “Caged Proton” — UV/VIS and IR Studies

@inproceedings{Fogel1993TitrationOP,
  title={Titration of Protonable Residues in Proteins by Flash Induced H+-Release from “Caged Proton” — UV/VIS and IR Studies},
  author={C A Fogel and F. Petry and Andreas Barth and Werner Maentele},
  year={1993}
}
Infrared spectroscopy has proven to be a valuable tool for the study of enzyme structure and function. Using differential techniques, molecular changes in the course of photoreactions and redox reactions have been studied (for a review see [1]). Recently, the use of photolabile substrate analogues, which can be released by a UV flash, has been reported for the study of ATPases [2,3]. This technique of photo-chemo-triggering a reaction can be used for many different enzymes (see Barth et al… CONTINUE READING