Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing

@article{Laski1986TissueSO,
  title={Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing},
  author={Frank A Laski and Donald C. Rio and Gerald M. Rubin},
  journal={Cell},
  year={1986},
  volume={44},
  pages={7-19}
}
We show that the germline specificity of P element transposition is controlled at the level of mRNA splicing and not at the level of transcription. In the major P element RNA transcript, isolated from somatic cells, the first three open reading frames are joined by the removal of two introns. Using in vitro mutagenesis and genetic analysis we demonstrate the existence of a third intron whose removal is required for transposase production. We propose that this intron is only removed in the… Expand
Drosophila P-element transcripts are incorrectly processed in tobacco
TLDR
A gene encoding the Drosophila P-element transposase was expressed in transgenic tobacco plants under transcriptional control of the Dosophila HSP70 promoter, providing an additional example in which RNA processing enzymes of plants utilize different mRNA processing signals than animals. Expand
Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron
P element transposition in Drosophila melanogaster is limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. WeExpand
Splicing of the Drosophila P element ORF2-ORF3 intron is inhibited in a human cell extract
TLDR
It is shown that a trans-acting factor in a human (HeLa) cell extract can inhibit splicing of the intron, suggesting that this regulatory mechanism is conserved from flies to humans. Expand
Regulated splicing of the Drosophila P transposable element third intron in vitro: somatic repression.
TLDR
Inhibition of IVS3 splicing in vitro could be correlated with binding of the 97-kD protein to 5' exon sequences, suggesting that one aspect of IVs3 tissue-specific splicing involves somatic repression by specific RNA-protein interactions. Expand
Developmentally Regulated Splicing of the Third Intron of P Element in Somatic Tissues in Drosophila Embryos
TLDR
The somatic tissues where the third intron is spliced out during embryogenesis is described, with reference to the stages when the splicing occurs, and a modification of the previous documentation that the complete processing of transcripts from P element takes place only in germ‐line cells is proposed. Expand
Evidence for Drosophila P element transposase activity in mammalian cells and yeast.
Drosophila P element transposase expression is limited to the germline by tissue-specific splicing of one of its three introns. Removal of this intron by mutagenesis in vitro has allowed both PExpand
Regulation of Drosophila P element transposition.
  • D. Rio
  • Biology, Medicine
  • Trends in genetics : TIG
  • 1991
TLDR
Drosophila P transposable elements are the best-studied family of eukaryotic non-retroviral transposons and has provided a unique system with which to study the control of DNA rearrangements and gene expression in metazoans. Expand
Negative regulation of P element excision by the somatic product and terminal sequences of P in Drosophila melanogaster
TLDR
A transient in vivo P element excision assay was used to test the regulatory properties of putative repressor-encoding plasmids in Drosophila melanogaster embryos and effectively repressed P excision in a dose-dependent manner at very low concentrations relative to somatically active transposase. Expand
Meiosis-specific RNA splicing in yeast
TLDR
This study reports a case of posttranscriptional regulation of a gene whose product is essential for meiosis, and genetic data suggest that the MER1 gene may also be responsible for splicing the transcript of at least one other gene. Expand
Structural basis for the regulation of splicing of a yeast messenger RNA
TLDR
An RNA structure within the transcript that is responsible for regulation of ribosomal protein L32 inhibits splicing by precluding the interaction of U1 RNA with the 5' splice site. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 49 REFERENCES
A trans-acting product needed for P factor transposition in Drosophila.
TLDR
A transposable genetic element of the P family in Drosophila melanogaster was found to be unstable in the presence of other P elements but stable in their absence, and this measure of activity was highly correlated with a type of female sterility normally associated with P activity. Expand
Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site
Early region IA of human adenoviruses encodes a function required for normal induction of early viral genes and virus-induced cell transformation. The region is expressed at early times as twoExpand
Analysis of P transposable element functions in drosophila
TLDR
A P-element derivative called Pc[ry], which carries the selectable marker gene rosy, but which acts like a nondefective, intact P element, is made, which suggests that a single polypeptide comprises the transposase. Expand
Effects of transposable element insertions on RNA encoded by the white gene of Drosophila
TLDR
It is shown that a mutation at each of two other loci that modulate the phenotype of the white-apricot insertion mutant are correlated with small but significant changes in the pattern of white transcripts. Expand
Transposition of cloned P elements into Drosophila germ line chromosomes.
TLDR
Recombinant DNA carrying the 3-kilobase transposable element was injected into Drosophila embryos of a strain that lacked such elements, and observations provide the basis for efficient DNA-mediated gene transfer in Dosophila. Expand
Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit β-globin gene
Only one of six point mutations of the sequence around one end of the larger of the introns of the rabbit β-globin gene seriously affects the normal removal of the intron and splicing of the gene.Expand
Effects of genomic position on the expression of transduced copies of the white gene of Drosophila.
TLDR
It is demonstrated that the mutant expression in Drosophila is caused by the surrounding chromosomal region into which the white gene has been inserted, and that flies in which white has been moved to one new location have an unusual mosaic phenotype. Expand
Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome
TLDR
A revertant of one of the white locus mutants has been found to result from precise excision of the P element, restoring the wild-type DNA sequence. Expand
Developmental variations in the splicing pattern of transcripts from the Drosophila gene encoding myosin alkali light chain result in different carboxyl-terminal amino acid sequences.
TLDR
The total sequence of the Drosophila melanogaster gene encoding the myosin light chain dissociated by alkali (MLC-ALK) has been determined and the pattern of introns and exons within the gene has been deduced. Expand
Vectors for P element-mediated gene transfer in Drosophila.
TLDR
This work has constructed and tested several new vectors for P element-mediated gene transfer and describes a genomic sequence arrangement that apparently arose by the transposition of a 54 kb composite P element from a tetramer plasmid. Expand
...
1
2
3
4
5
...