Tissue-specific tagging of endogenous loci in Drosophila melanogaster.

  title={Tissue-specific tagging of endogenous loci in Drosophila melanogaster.},
  author={Kate Koles and Anna R Yeh and Avital A Rodal},
  journal={Biology open},
  volume={5 1},
Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners and/or developmental windows of gene expression. To address these limitations, we have developed a method called T-STEP (tissue-specific tagging of endogenous proteins… CONTINUE READING
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