Tissue-specific nuclear architecture and gene expession regulated by SATB1

@article{Cai2003TissuespecificNA,
  title={Tissue-specific nuclear architecture and gene expession regulated by SATB1},
  author={Shutao Cai and Hye-jung Han and Terumi Kohwi-Shigematsu},
  journal={Nature Genetics},
  year={2003},
  volume={34},
  pages={42-51}
}
Eukaryotic chromosomes are packaged in nuclei by many orders of folding. Little is known about how higher-order chromatin packaging might affect gene expression. SATB1 is a cell-type specific nuclear protein that recruits chromatin-remodeling factors and regulates numerous genes during thymocyte differentiation. Here we show that in thymocyte nuclei, SATB1 has a cage-like 'network' distribution circumscribing heterochromatin and selectively tethers specialized DNA sequences onto its network… 
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407 Citations
Highly Influential Citations
26
Background Citations
185
Methods Citations
13
Results Citations
4

407 Citations

Deeply hidden genome organization directly mediated by SATB1
TLDR
A modified ChIP-seq protocol is developed that stringently purifies genomic DNA only with its directly-associated proteins and unmasked previously-hidden BURs as direct SATB1 targets genome-wide, revealing TAD-independent chromatin folding mediated by BUR sequences which serve as genome architecture landmarks for direct targeting by cell type-specific gene regulator, SATB 1.
SATB1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes
TLDR
RNA interference shows that on cell activation, SATB1 is required not only for compacting chromatin into dense loops at the 200-kb cytokine locus but also for inducing Il4, Il5, Il13 and c-Maf expression.
The third dimension of gene regulation: organization of dynamic chromatin loopscape by SATB1.
Nuclear Matrix Binding Regulates SATB1-mediated Transcriptional Repression*
Special AT-rich binding protein 1 (SATB1) originally was identified as a protein that bound to the nuclear matrix attachment regions (MARs) of the immunoglobulin heavy chain intronic enhancer.…
SATB1-mediated functional packaging of chromatin into loops.
Phosphorylation-dependent regulation of SATB1, the higher-order chromatin organizer and global gene regulator.
TLDR
Methods for overexpression and purification of full length SATB1 protein and for its in vitro phosphorylation are described and a functional assay to monitor the effect of phosphorylated on transcription activity of SATB 1 in vivo is described using MAR-linked reporter assay, in the presence and absence of PKC inhibitors.
A long-range chromatin interaction regulates SATB homeobox 1 gene expression in trophoblast stem cells
TLDR
These findings indicate that trophoblast-specific Satb1 expression is regulated by long-range chromatin looping of an enhancer that interacts with ELF5 and SATB proteins.
A Nuclear Targeting Determinant for SATB1, a Genome Organizer in the T Cell Lineage
TLDR
The N-terminus of human SATB1 was found to be essential for the nuclear localization of the protein, and fusing residues 20-40 to a cytoplasmic green fluorescence protein (GFP) fused to pyruvate kinase (PK) was sufficient to quantitatively translocate the pyruVate kinases into the nucleus.
The impact of histone post-translational modifications on developmental gene regulation
TLDR
This review examines the molecular processes regulating site-specific histone acetylation, methylation and phosphorylation with an emphasis on how these processes underpin differentiation-regulated transcription.
Tetramerization of SATB1 is essential for regulating of gene expression
TLDR
Whether SATB1’s oligomerization is critical to its function as a global repressor of gene expression in vivo is illustrated to illustrate.
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