Tissue-specific expression of a BAC transgene targeted to the Hprt locus in mouse embryonic stem cells.

@article{Heaney2004TissuespecificEO,
  title={Tissue-specific expression of a BAC transgene targeted to the Hprt locus in mouse embryonic stem cells.},
  author={Jason D Heaney and Ashley N. Rettew and Sarah K. Bronson},
  journal={Genomics},
  year={2004},
  volume={83 6},
  pages={1072-82}
}
The hypoxanthine phosphoribosyltransferase (Hprt) locus has been shown to have minimal influence on transgene expression when used as a surrogate site in the mouse genome. We have developed a method to transfer bacterial artificial chromosomes (BACs) as a single copy into the partially deleted Hprt locus of embryonic stem cells. BACs were modified by Cre/loxP recombination to contain the sequences necessary for homologous recombination into and complementation of the partially deleted Hprt… CONTINUE READING