Tissue fractionation and catecholamines--IV. Adenosine triphosphatase in chromaffin granules: a distribution artefact.


Tissue fractionation was used as an analytical tool to study the subcellular distribution of an adenosine triphosphatase activated by Mg2+ in adrenal medullae of the pig and ox and in whole adrenals of the rat. By measuring adenosine triphosphatase and various enzymes in the fractions obtained by differential centrifugation, the distribution pattern of… (More)