Tissue disruption and extraction methods for metabolic profiling of an invertebrate sentinel species

@article{Liebeke2011TissueDA,
  title={Tissue disruption and extraction methods for metabolic profiling of an invertebrate sentinel species},
  author={Manuel Liebeke and Jacob G Bundy},
  journal={Metabolomics},
  year={2011},
  volume={8},
  pages={819-830}
}
Metabolic profiling of tissues needs special attention, because the compartmentalization of cellular constituents will be abolished by sample homogenization. This loss of partitioning leads to protein and metabolite instability in extracts, and therefore metabolite extraction protocols need to ensure very rapid inactivation of macromolecules as well as solubilization of metabolites. There are many published methods for tissue metabolome analysis, but no universally accepted standard, and a lack… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 52 references

Metabolite extraction

F M., P. J. Oefner
2011

Save our soils

Nature • 2011
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