Tissue culture of epithelial cells from urine. I. Serum-free growth of cells from newborn infants.

@article{Hintz1984TissueCO,
  title={Tissue culture of epithelial cells from urine. I. Serum-free growth of cells from newborn infants.},
  author={D S Hintz and Mary Ann Sens and Margaret Q Jenkins and Donald A Sens},
  journal={Pediatric pathology},
  year={1984},
  volume={2 2},
  pages={153-63}
}
The growth of epithelial cells from the urine of newborn infants was improved by the use of serum-free medium and a collagen type 1 matrix present on the growth surface of the culture vessel. The optimal concentrations and components of the serum-free medium consisted of a 1:1 mixture of Dulbecco's Modified Eagles' medium and Ham's F-12 medium supplemented with insulin (5 micrograms/ml), transferrin (5 micrograms/ml), selenium (5 ng/ml), and hydrocortisone (1 X 10(-7) M). The use of this medium… CONTINUE READING

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