Tissue culture of adult human neurons

@article{Kim1979TissueCO,
  title={Tissue culture of adult human neurons},
  author={Seung U. Kim and Kenneth G. Warren and Madhu Kalia},
  journal={Neuroscience Letters},
  year={1979},
  volume={11},
  pages={137-141}
}
Dissociated neurons from adult human trigeminal and superior cervical ganglia were cultured in vitro for more than 2 months. Immediately after dissociation by incubation in 0.06% collagenase for 15--18 h, the cultures consisted of single neurons or clumps of neurons and degenerating fragments of myelinated or non-myelinated axons. After 7--10 days, bipolar Schwann cells, large neurons and fine nerve fibers were observed. Electron microscopic examination of these neurons revealed all the… Expand
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