Tissue culture and Agrobacterium-mediated transformation of watercress

@article{Jin2004TissueCA,
  title={Tissue culture and Agrobacterium-mediated transformation of watercress},
  author={Rong-Guan Jin and Yong-Biao Liu and Bruce E Tabashnik and Dulal Borthakur},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={58},
  pages={171-176}
}
Adventitious shoot regeneration could be obtained from more than 80% of the calluses initiated from stem explants of watercress (Rorippa nasturtium-aquaticum) by using an induction medium and a shoot regeneration medium. The induction medium contained 1.15 μM 2,4-dichlorophenoxyacetic acid and 5 μM thidiazuron; the shoot regeneration medium was composed of 0.5 μM thidiazuron and 2.25 μM 6-benzylaminopurine. This regeneration procedure was incorporated into an Agrobacterium-mediated… CONTINUE READING
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