Tissue- and cell-specific distribution of creatine kinase B: A new and highly specific monoclonal antibody for use in immunohistochemistry

@article{Sistermans1995TissueAC,
  title={Tissue- and cell-specific distribution of creatine kinase B: A new and highly specific monoclonal antibody for use in immunohistochemistry},
  author={Erik A Sistermans and Yvette J. M. de Kok and Wilma Peters and Leo A. Ginsel and Paul H. K. Jap and B. Wieringa},
  journal={Cell and Tissue Research},
  year={1995},
  volume={280},
  pages={435-446}
}
A synthetic 17-mer peptide corresponding to an unique sequence in the amino-terminal region of human creatine kinase B was used to raise a new and highly B-subunit-specific monoclonal antibody, CK-BYK/21E10. We show here that the monoclonal antibody is suitable for immunohistochemistry of unfixed frozen sections as well as formaldehyde- or Bouin-fixed, paraffin-embedded sections of human, rabbit, and mouse tissues. Moreover, in the study of cell- and tissue-specific distribution patterns… CONTINUE READING