Timing of neuronal and glial ultrastructure disruption during brain slice preparation and recovery in vitro.

@article{Fiala2003TimingON,
  title={Timing of neuronal and glial ultrastructure disruption during brain slice preparation and recovery in vitro.},
  author={John C. Fiala and Sergei A Kirov and Marcia D Feinberg and Lara J Petrak and Priya George and C Alex Goddard and Kristen M Harris},
  journal={The Journal of comparative neurology},
  year={2003},
  volume={465 1},
  pages={90-103}
}
Hippocampal slices often have more synapses than perfusion-fixed hippocampus, but the cause of this synaptogenesis is unclear. Ultrastructural evidence for synaptogenic triggers during slice preparation was investigated in 21-day-old rats. Slices chopped under warm or chilled conditions and fixed after 0, 5, 25, 60, or 180 minutes of incubation in an interface chamber were compared with hippocampi fixed by perfusion or by immersion of the whole hippocampus. There was no significant… CONTINUE READING