Time-resolved immunofluorometric assay of trypsin-2 complexed with alpha 1-antitrypsin in serum.

@article{Hedstrm1994TimeresolvedIA,
  title={Time-resolved immunofluorometric assay of trypsin-2 complexed with alpha 1-antitrypsin in serum.},
  author={Johan Hedstr{\"o}m and Jari T Leinonen and V S Sainio and Ulf Hakan Stenman},
  journal={Clinical chemistry},
  year={1994},
  volume={40 9},
  pages={1761-5}
}
We developed a sensitive time-resolved immunofluorometric assay (IFMA) for trypsin-2 complexed with alpha 1-antitrypsin (AAT). We used a trypsin-2-specific monoclonal antibody on the solid phase and a europium-labeled polyclonal antibody to AAT as tracer. The detection limit is 0.05 microgram/L and the range of linearity extends to 100 micrograms/L. We compared the clinical utility of the trypsin-2-AAT assay with that of free trypsinogen-2 and amylase in serum by studying 120 healthy subjects… CONTINUE READING

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