Time-resolved fluoroimmunoassay for ractopamine in swine tissue.

  title={Time-resolved fluoroimmunoassay for ractopamine in swine tissue.},
  author={Jianzhong Shen and Zhen Zhang and Yan Yao and Weimin Shi and Yibing Liu and Suxia Zhang},
  journal={Analytical and bioanalytical chemistry},
  volume={387 4},
This study describes the development and validation of a time-resolved fluoroimmunoassay (TR-FIA) for screening ractopamine (RAC) in swine tissue. The method is based on the direct competitive-type immunoassay using europium-labeled anti-RAC monoclonal antibody as a tracer and RAC-ovalbumin as a solid-phase antigen. When RAC was spiked at levels of 1-10 microg kg(-1), recoveries ranged from 88.2 to 118.5% for swine liver and muscle with coefficients of variation from 7.1 to 20.5%. The detection… CONTINUE READING