Time-resolved fluorescence spectroscopy and imaging of DNA labeled with DAPI and Hoechst 33342 using three-photon excitation.

@article{Lakowicz1997TimeresolvedFS,
  title={Time-resolved fluorescence spectroscopy and imaging of DNA labeled with DAPI and Hoechst 33342 using three-photon excitation.},
  author={J. R. Lakowicz and Ignacy Gryczynski and H M Malak and M V Schrader and Philip Engelhardt and Hiroyuki Kano and Stefan W. Hell},
  journal={Biophysical journal},
  year={1997},
  volume={72 2 Pt 1},
  pages={
          567-78
        }
}
We examined the fluorescence spectral properties of the DNA stains DAPI (4',6-diamidino-2-phenylindole, hydrochloride) and Hoechst 33342 (bis-benzimide, or 2,5'-bi'1H-benzimidazole2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazi nyl)) with two-photon (2h nu) and three-photon (3h nu) excitation using femtosecond pulses from a Ti:sapphire laser from 830 to 885 nm. The mode of excitation of DAPI bound to DNA changed from two-photon at 830 nm to three-photon at 885 nm. In contrast, Hoechst 33342… CONTINUE READING

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