Time-resolved fluorescence receptor assay for benzodiazepines

  title={Time-resolved fluorescence receptor assay for benzodiazepines},
  author={T. Takeuchi and Midori Yoshida and Y. Kabasawa and R. Matsukawa and E. Tamiya and I. Karube},
  journal={Analytical Letters},
Abstract A novel non-isotopic receptor binding technique for the detection of benzodiazepines is described. A benzodiazepine labeled with europium chelate was prepared and employed as a labeled ligand, and time-resolved measurements of the long lifetime fluorescence of europium chelate allowed avoidance of interference due to proteins in the receptor preparation. Experimental results demonstrate a sigmoid inhibition curve, with binding of the labeled ligand inhibited by comparable… Expand
16 Citations
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