Time-resolved fluorescence of 2-aminopurine in DNA duplexes in the presence of the EcoP15I Type III restriction-modification enzyme.

@article{Ma2014TimeresolvedFO,
  title={Time-resolved fluorescence of 2-aminopurine in DNA duplexes in the presence of the EcoP15I Type III restriction-modification enzyme.},
  author={Long Ma and Xiaohua Wu and Geoffrey G. Wilson and Anita C. Jones and David T. F. Dryden},
  journal={Biochemical and biophysical research communications},
  year={2014},
  volume={449 1},
  pages={120-5}
}
EcoP15I is a Type III DNA restriction and modification enzyme of Escherichia coli. We show that it contains two modification (Mod) subunits for sequence-specific methylation of DNA and one copy of a restriction endonuclease (Res) subunit for cleavage of DNA containing unmethylated target sequences. Previously the Mod2 dimer in the presence of cofactors was shown to use nucleotide flipping to gain access to the adenine base targeted for methylation (Reddy and Rao, J. Mol. Biol. 298 (2000) 597… CONTINUE READING
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  • F. W. Schwarz, J. Tóth
  • van Aelst, G. Cui, S. Clausing, M.D. Szczelkun, R…
  • 2013

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