Time-resolved fluorescence biosensor for adenosine detection based on home-made europium complexes.

@article{Huang2011TimeresolvedFB,
  title={Time-resolved fluorescence biosensor for adenosine detection based on home-made europium complexes.},
  author={Da-Wei Huang and Cheng-Gang Niu and Guang-Ming Zeng and Min Ruan},
  journal={Biosensors & bioelectronics},
  year={2011},
  volume={29 1},
  pages={178-83}
}
In this protocol, the authors report a time-resolved fluorescence biosensor based on home-made europium complexes for highly sensitive detection of small molecules using adenosine as a model analyte. The fluorophore that used is europium complexes. Its signal can be measured in a time-resolved manner that eliminates most of the unspecific fluorescent background. The amino modified aptamer probe, which is designed to specifically recognize adenosine, is combined to the aldehyde-group modified… CONTINUE READING